High throughput organoids: 5000+ spheroids in a standard multiwell plate
Explore the potential of 4Dcell’s technology to form functional and reproducible spheroids
> READY TO USE
Plate your cells directly on the microstructured substrates
> MAINTAIN +95% OF SPHEROIDS, UNIFORM SIZE AND SHAPE
Keep your spheroids alive, with <6% variation of the spheroid’ size.
> NO SPHEROIDS DETACHMENT DURING MEDIUM CHANGE OVER WEEKS
The patented anchoring point technology keeps the spheroids in each microwell during medium change
> PREDICTABLE LOCATION AND EASY TO IMAGE
Cell aggregates are located in predictable positions enabling automated image acquisition
4Dcell offers 6, 12 and 24 well plates containing hydrogel-based microwell structures specially designed for the spontaneous formation of uniform spheroids. The anti-adhesive properties of the hydrogel facilitates rapid self-assembly of cell aggregates into compact spheroids.
The adhesive bottom surface of each microwell anchors the spheroids in place. This prevents the loss of spheroids during typical manipulations (medium exchange, drug addition, etc), and makes the location of each spheroid predictable. As the hydrogel is optically transparent it is fully compatible with conventional microscopy.
FEATURES & BENEFITS
4DCELL 6 WELL PLATES WITH MICROWELLS FOR SPHEROIDS’ ASSEMBLY:
6, 12 and 24 well plates with glass bottom ( #1.5, 0.16 – 0.19 mm), micro-well size 14 mm or 10 mm (96 well plate under development)
Each macrowell has 91 individual microwells where spheroids are assembled
The microstructured substrates are made of biocompatible polyethylene glycol gel
Optionally the microwells can be coated with an ECM matrix to increase the adherence of the spheroid to the contact (attachment) point
The gels are provided in a water based solution that keeps their mechanical and optical properties
Mesenchymal Stem cells (MSCs) seeded in a 3D structured hydrogel designed for controlled spheroid formation (4Dcell SmartSphero plates).
Seeding density: 3000 cells/microwell. The video shows the initial stages of spheroid formation, including cell aggregation and spheroid compaction, followed by spheroid growth in the days following compaction due to cell proliferation.
Structure and dimensions of the plate and macrowell
Structure and dimensions of the microstructured gel and microwell
EXAMPLE OF LOSS OF SPHEROIDS AFTER MANIPULATION (WITH AND WITHOUT CONTACT POINTS):
CANCER
> Signaling pathways
> Immune activation
> Tissue Structure
DRUG SCREENING
REGENERATIVE MEDICINE
TOXICOLOGY
Spheroids – Mesenchymal stem cells (MSCs) labeled with GFP after 72 hours.