Macrophage polarization assay
Modulation of macrophage phenotype by cell shape
4DCELL DEVICE
READ-OUTS
Regulation of the functional phenotype
STANDARD CULTURE LIMITATION
Up to now, it was thought that cytokines and chemokines were the primary regulators of macrophage behavior. However, biophysical cues are also involved in this process
MACROPHAGE POLARIZATION ASSAY BENEFITS
Recent studies showed that adhesive cues might modulate proinflammatory versus prohealing activation.
With 4Dcell micropatterns, you can induce an elongation of cells that promote a prohealing phenotype.
EXAMPLE
Elongation of macrophages by micropatterning
Cell elongation promotes macrophage polarization toward a prohealing M2 phenotype (increase of arginase-1 level) without influencing inflammatory activation (stable low level of iNOS).
