Cell interaction assay
2D interaction of non-adherent (immune) cells
4DCELL DEVICE
READ-OUTS
Interaction between cells
CELL TYPE
All non-adherent cells, immune cells, etc.
STANDARD CULTURE LIMITATION
Non-adherent immune cells poorly migrate on flat surfaces, which limits their interaction with other cells.
In addition, there is an enormous difficulty in qualifying immune cell migration and interaction, since these cells depend on non-adherent confined migration. These events cannot be reproduced in a common petri-dish.
IMMUNE SYSTEM IN A WELL BENEFITS
It is easier to observe non-adherent (immune) cells in 2D confined spaces than in a 3D matrix. This configuration leads to an increased probability of cellular interaction.
EXAMPLES
In a confined environment non-adherent immune cells interact at a faster rate [1].
(A) Confinement principle: cells are immobilized between two parallel surfaces with a controlled height.
(B) Tracking of cell nuclei position. Results show that cells move more and faster in a confined space enabling to meet neighbouring cells. Scale bars: 100 μm.