3D Cardiomyocytes maturation assay
Assessing the beating of iPSC-CMs
4DCELL DEVICE
Coverslips coated with microstructured gels
READ-OUTS
Cardiac muscle structure observation and contractility force measurement
STANDARD CULTURE LIMITATION
In standard cultures, cardiomyocytes derived from pluripotent stem cells have random shapes and are not organized thus showing immature properties.
3D CARDIOMYOCYTES MATURATION ASSAYS
When cultured on 3D gels, hiPSC-CMs better mimic the real cardiac muscle structure and functions, thus leading to a more efficient maturity of iPSC-CMs.
EXAMPLE
Using a microgroove-patterned hydrogel, cardiomyocytes are organized for a better cell characterization (A). In addition, when comparing unpatterned cells with patterned ones on different groove sizes, the contraction performance is increased with the cells confinement (B)(C).
