3D Liver canaliculi assays
Spheroid formation in 3D gels
4DCELL DEVICE
READ-OUTS
Identification, quantification and analysis of bile canaliculi
STANDARD CULTURE LIMITATION
Cultured in a 2D environment, cells loose rapidly their phenotypic expression and their functions.
3D LIVER CANALICULI ASSAYS
A 3D environment is more relevant for cell culture because it enables to reflect in vivo physiology of cells.
4Dcell offers soft 3D gels for the fulfilment of a cell culture enabling spheroid formation to better mimic in vivo conditions.
EXAMPLE
Accumulation of F-actin, MRP2 and MDR1, specific markers of the canalicular plasma membrane, have been detected in low-density HepaRGTM cultures using wood-derived nanofibrillar cellulose (NFC) and hyaluronan-gelatin (HG) hydrogels after spheroid formation. It has been compared to a 2D cell culture where the activity of MRP2 and MDR1 is lower.
Spheroid formation in a soft substrate versus flat substrate