MICROCHANNELS FABRICATION KIT

WHICH KIT FITS YOUR NEEDS?

If you never made any microchannels:

Microchannels Station (microchannels box + plasma cleaner)

If you already have a binding tool (for example a plasma cleaner):
Microchannels Box (supplies box + microchannels mold)

If you have all the equipment but want to access the best chemicals:
Supplies Box (silicone precursors, glass bottom petri dishes and solutions)

Each element can also be ordered separately.

WHAT DO YOU NEED TO MAKE YOUR OWN MICROCHANNELS?

To manufacture 100 chips, each 4DCell microchannels kit contains:

> 1 x Silicone precursors to make 100 chips

> 2 x graduated plastic cups to measure the quantity of elastomer needed

> 1 x glass spatula to homogenize the PDMS with its curing agent

> 4 x individual molds to shape the silicone into the upper part of the chip containing the microchannels

> 1 x Scalpel to unmold the chip

> 1 x Thin glass bottom 35 mm petri dishes to make the lower part of the microchannels, and to observe cells with a microscope

> 1 x punches round to cut 3 mm holes into the chip

> 1 x Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin)

> 1 x Plasma cleaner to activate the surface of the chip and allow its bonding to the glass

The mold on which the microchannels shapes are defined is the key element.
Several series of shapes are available, for migration assay with various sizes, shapes and profiles.
We put our expertise at your disposal to help you make your choice.

MICROCHANNELS’ DIMENSIONS

The 3D shape and stress conditions imposed to your cells depend on the microchannels size. Each chip has 6 ranges of microchannels and 9 access ports.

MICROCHANNELS WITH CONSTRICTIONS

Example of a 4Dcell microchannel with a constriction of 1.5 µm width.