Skeletal Muscle Cells Assay
Alignment, organization and shape standardization
4DCELL DEVICE
READ-OUTS
Alignment and organization of skeletal muscle cells
STANDARD CULTURE LIMITATION
In vivo, cells are highly organized to form structures, tissues, and organs – and that structure helps determine function. Conventional 2D cell culture conditions – in which cells are given a boundless surface on which to grow in any orientation – don’t reflect the cell’s natural microenvironment.
SKELETAL MUSCLE CELLS ASSAY
When cultured in a substrate with line adhesive cues, skeletal muscle cells get organized and acquire an elongated shape. Using 4Dcell assay, skeletal myoblasts are more easily differentiated.
EXAMPLE
Micropatterned culture with myoblasts [1]
C2C12 cells are seeded on micropatterned lines to induce differentiation
Immunofluorescence of cells [1]
Nuclei in blue and Myosine Heavy Chain (MHC) in green in C2C12 cells cultured on micropatterned lines. Organization and alignment of cells are required to induce cell contractility. By improving the cell alignment, cell differentiation leading to cell contractility will occur rapidly.
