Quantification of cell migration speed, persistence and diffusion coefficient
All migrating cells (e.g. immune cells)
Non-adherent immune cells poorly migrate on flat surfaces, which can limit the analysis of their behavior. In addition, the 2D random walk of a cell is difficult to analyse.
When confined, immune cells show restored migration behaviors and can migrate in 1D, 2D or 3D spaces.
This makes their observation and analysis with a microscope easier than within a 3D matrix, enabling the quantification of their exact cell speed, persistence and diffusion coefficient.
Migration of Dendritic cells (DCs) in a 400 μm wide fibronectin coated microchannel. Measurement of speed for wild type DCs versus li (antigen) regulated DCs [2]: