4DCELL DEVICE
READ-OUTS
Quantification of cell migration speed, persistence and diffusion coefficient
CELL TYPE
All migrating cells (e.g. immune cells)
STANDARD CULTURE LIMITATION
Non-adherent immune cells poorly migrate on flat surfaces, which can limit the analysis of their behavior. In addition, the 2D random walk of a cell is difficult to analyse.
QUANTITATIVE CELL MIGRATION ASSAY BENEFITS
When confined, immune cells show restored migration behaviors and can migrate in 1D, 2D or 3D spaces.
This makes their observation and analysis with a microscope easier than within a 3D matrix, enabling the quantification of their exact cell speed, persistence and diffusion coefficient.
EXAMPLE
Migration of Dendritic cells (DCs) in a 400 μm wide fibronectin coated microchannel. Measurement of speed for wild type DCs versus li (antigen) regulated DCs [2]:
