Neuronal network in a well
Control of neurons connectivity
4DCELL DEVICE
READ-OUTS
Trafficking, outgrowth, propagation of action potentials and other signals
STANDARD CULTURE LIMITATION
The differentiation of neuronal cells from iPSC results in clusters of neurons, which precludes the analysis of individual neurons and defined neuronal networks.
NEURONAL NETWORK IN A WELL
When cultured in substrates with adhesive cues, neuronal networks appear in an organized fashion, enabling to study cellular trafficking, mechanisms that require assessment of individual neurons and specific network connections. Besides this, these assays support long-term stability of cultured neurons.
EXAMPLE
Improvement of neuronal network formation in iPSC-derived neurons cultured in patterned coverslips (round/flower shape) when compared with a culture in a common substrates [1].
