MICROPATTERNED COVERSLIPS
Patterned slides for immunofluorescence observation
Patterned slides for immunofluorescence observation
4Dcell offers 24 mm round micropatterned coverslips ready to be applied in cell culture experiments.
Several standard micropattern shapes available such as triangles, lines, rectangles, squares, etc.
Plate the cells on the patterned coverslip and observe them directly
Place your slide on the imaging system suitable for your study
The covalent bonds formed between the anti-adhesive coating and the glass coverslip enable storing for several months at room temperature
HOW TO HANDLE YOUR MICROPATTERNED COVERSLIPS
4Dcell micropatterned coverslips are shipped in individual classic 35 mm Petri dishes in plastic bags (aseptic conditions).
Available in sets of 20 or 50 units to allow optimization of your experimental conditions with only one kit. You can choose various micropatterned shapes to compose your set.
Compatible with high resolution microscopy, phase contrast, immunofluorescence, etc.
The coverslips may be combined with perfusion chamber.
Disks
Lines
Triangles
Squares
Rectangles
Grids
Customized shapes
Each type of micropatterned coverslip (disks, rectangles, squares, etc.) is arranged as described in the scheme bellow (e.g.: disks).
In each standard coverslip, there are seven zones with distinct pattern sizes (ranging from 10 µm to 100 µm). There is an eighth zone where all the sizes are placed together.
Standard positionning of micropattern areas
Oxidation resistance anti-adhesive polymer which is less sensitive to oxidation than a classical polymer and has the same type of covalent bonding to the glass slide. After plated you can keep your cells in the patterns for more than a week. Non used coverslips can be stored up to 6 months.
CANCER
> Migration of cancer cells (line patterns)
> Cell shape standardization
> Migration of somatic and cancer cells
> Cell shape standardization
> Cardiomyocyte beating properties
> Neural network
> Cell nucleus integrity
> Nuclear plasticity
> Neural network
> Cell shape standardization
> Standardized connectivity between neurons
> Spatial autoorganization of macromolecules (at the microscale)
hPSC-CM on line micropatterns of 10 to 70 µm width imaged by fluorescence microscopy.
Dussaud S., Jouve C., Hulot J.S., 2018.
Image of micropatterned HeLa cells in lines of 100 to 10 µm width, 4Dcell lab (2018).
Image of micropatterned cells in lines (labelled by fluorescence) obtained with a 4Dcell photomask.
Hôpital Saint Louis, experiment run with Dr. Benoit Vianay.