‘DYNAMIC’ MICROPATTERNED COVERSLIPS
Patterned slides ready to be plated with cells for cell migration assays
Patterned slides ready to be plated with cells for cell migration assays
The micropatterned Petri dishes are 35 mm glass bottom Petri dishes printed with micropatterns.
They are ready to use, you just need to plate your cells on the glass substrate, wait for them to be patterned and observe them migrating on the substrate.
Plate the cells directly on the patterned coverslip and observe them directly
Observe the cells through the optically transparent micropatterned glass substrate
The covalent bonds formed between the anti-adhesive coating and the glass coverslip enable storing for several months at room temperature
The cells are first patterned in cell-adhesive regions and do not adhere outside of these regions. The surrounding anti-adhesive regions can be rendered adhesive by adding a single reagent onto the substrate : this induces cell migration into the newly adhesive regions.
The 4Dcell micropatterned dishes are provided in individual sterile bags (aseptic conditions). Available in sets of 20 or 50 dishes to allow optimization of your experimental conditions with only one kit.
Compatible with high resolution microscopy, phase contrast, immunofluorescence, etc.
The coverslips may be combined with a perfusion chamber.
As with regular micropatterned coverslips and dishes, the available shapes are:
Disks
Lines
Triangles
Squares
Rectangles
Grids
Customized shapes
Each type of micropatterned coverslip (disks, rectangles, squares, etc.) is arranged as described in the scheme bellow (e.g.: disks).
In each standard coverslip, there are seven zones with distinct pattern sizes (ranging from 10 µm to 100 µm). There is an eighth zone where all the sizes are placed together.
Standard positionning of micropattern areas
A HIGHLY STABLE NON-ADHESIVE COVALENT COATING
Long term cell culture anti-adhesive polymer which is the best in terms of anti-fouling treatment and bonding to the glass slide. Observable by phase contrast microscopy. This is the best option for long term cell culture on micropatterns (more than a week of cell culture).
he reagent for switching the coating from ‘non-adhesive’ to ‘ adhesive’ and activate cell migration is shipped in aliquots of 50 ug lyophilized powder. Once dissolved in its appropriate solvent, it can be stored at -20°C.
Time lapse of Mesenchymal Stem Cells (MSCs) migration on 4Dcell dynamic micropatterns
The cells are initially patterned in 500 µm disks. At t0, the cells are released from their patterns and migrate while maintaining a circular shape.