The 4Dcell pre-coated slides are 24 mm slides treated with an anti-fouling reagent for the purpose of micropatterning experiment. They are ready to be patterned.
You can choose between 3 anti-adhesive molecules.
The pre-coated slides are packaged into petri dishes in sterile individual bags.
4Dcell offers 3 options of covalent coatings:
- Standard anti-adhesive polymer with the same anti-fouling effect than traditional PLL-g- PEG, but with a more stable bonding to the glass slide (covalent).
- Oxidation resistance anti-adhesive polymer which is less sensitive to oxidation and has the same type of covalent bonding to the glass slide.
- Long term cell culture anti-adhesive polymer which is the best in terms of anti-fouling treatment and bonding to the glass slide. Observable by phase contrast microscopy. This is the best option for long term cell culture on micropatterns (more than a week of cell culture).
The Micropatterned Dish provides a wide range of applications. Study and measure:
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Cell morphology
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Multicellular interactions: tissue assembly and morphogenesis
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Regulation of cell size, mitosis and meiosis
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Mechanics of cell Division and cytokinesis
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Membrane shaping
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Sub-cellular organisation
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Mechanical coupling from nucleus to extracellular matrix
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Border dynamics: envelope organization and remodeling
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Organelles in metabolism and stress responses
- Organelle morphogenesis, targeting, and distribution
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Cytoskeletal and actin dynamics
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Video of cells developing on the long term cell culture polymer micropatterns.
Dr Martial Balland, Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier.
Image of micropatterned cells in lines (labelled by fluorescence) obtained with a 4Dcell photomask
Hôpital Saint Louis, experiment run with Dr. Benoit Vianay