4DCELL DEVICE

Micropatterns

READ-OUTS

Regulation of the functional phenotype

STANDARD CULTURE LIMITATION

Up to now, it was thought that cytokines and chemokines were the primary regulators of macrophage behavior. However, biophysical cues are also involved in this process

MACROPHAGE POLARIZATION ASSAY BENEFITS

Recent studies showed that adhesive cues might modulate proinflammatory versus prohealing activation.

With 4Dcell micropatterns, you can induce an elongation of cells that promote a prohealing phenotype.

EXAMPLE

Elongation of macrophages by micropatterning 

Cell elongation promotes macrophage polarization toward a prohealing  M2 phenotype (increase of arginase-1 level) without influencing inflammatory activation (stable low level of iNOS).

REFERENCE

McWhorter, F. Y., et al. (2013). Proceedings of the National Academy of Sciences, 110(43), 17253-17258.