4DCELL DEVICE

Cell confiner

READ-OUTS

Attraction and interaction between cells, quantification of cell migration.

CELL TYPE

Immune cells

STANDARD CULTURE LIMITATION

Non-adherent immune cells migrate poorly on flat surfaces which can limit the analysis of their behavior.

IMMUNE SYSTEM IN A WELL BENEFITS

Confined between two surfaces (2D), immune cells show restored migration behavior. In these conditions, cells end up meeting more frequently thus enabling their observation and analysis easier than within a 3D matrix.

EXAMPLES

Migration of mice bone marrow-derived dendritic cells (DCs) in fibronectin coated substrates [3]:

DCs in a confinement assay shows a diffusion coefficient 5 times greater than when plated in common adhesive substrates (substrates coated with extracellular matrix).

REFERENCES

[1] Liu, Y-J., et al., Cell. 2015 Feb 12;160(4):659-672

[2] Maiuri, P., et al., Cell. 2015 Apr 9;161(2):374-86

[3] Heuzé, ML., et al., Immunol Rev. 2013 Nov;256(1):240-54