Perfuse 2000+ organoids in a standard multiwell plate
A ready to use long term cell culture solution which transforms your cells into stable, functional and reproducible vasculorized organoids
A ready to use long term cell culture solution which transforms your cells into stable, functional and reproducible vasculorized organoids
> MAINTAIN +95% OF SPHEROIDS, UNIFORM SIZE AND SHAPE
Keep your spheroids alive, with <6% variation of the spheroid’ size.
> MEDIUM PERFUSION WITHOUT SPHEROID LOSS OVER WEEKS
The patented anchoring point technology keeps your spheroids in each microwell during perfusion
> COMPATIBLE WITH HIGH RESOLUTION IMAGING
The whole system fits on your microscope, perfect for organoid tracking and automated image acquisition.
> VASCULARIZED IMMUNOCOMPETENT MODEL
The patented microfluidic mutliwell technology allows interconnection of adjacent wells to circulate drugs and immunocompetent cells through endothelium
A unique assay to test your drugs on reconstructed vascularized spheroids.
The microwell’s adhesive bottom surface technology anchors uniform spheroids in a transparent hydrogel fully compatible with live imaging.
The Microfluidic Perfusion Technology®, creates a membrane free functional vascular interface on top of the Spheroids by applying a constant shear stress to differentiate the endothelium layer.
This unique system preserves your 91 spheroids in each microwell with a permanent culture medium flow (supporting constant nutrient supply) over weeks and allows to image in real time the effect of your drug or immunocompetent therapies.
These combined technologies allow direct observation of up to 2184 spheroids in parallel during several weeks on a 24 well plate under optimal culture conditions.
FEATURES & BENEFITS
24 WELL PLATES WITH MICROWELLS FOR SPHEROIDS’ ASSEMBLY, UNDER PERFUSION
Perfusion adaptor
Transparent polymer, guaranteeing no autofluoresence
There is only a few microliters of dead volumes, avoiding a high dilution factor
Can perfuse from 350 µL to 850 µL per well (in a 24 multiwell plate)
Air-tight closure to avoid contamination
Principle of the perfused organoid
Organoid plate
24 well plates with glass bottom ( #1.5, 0.16 – 0.19 mm), micro-well size 14 mm or 10 mm
Each well contains 91 individual microwells where spheroids are nestled and anchored
The microstructured substrates are made of biocompatible polyethylene glycol gel
The microwells can be coated with an ECM matrix to increase the adherence of the spheroid to the contact (attachment) point
The gels are provided in a water based solution that maintains their mechanical and optical properties
Principle of the spheroid/organoid assembly
Structure and dimensions of the plate and microwell
Structure and dimensions of the the microstructured gel and microwell
EXAMPLE OF LOSS OF SPHEROIDS AFTER MANIPULATION (WITH AND WITHOUT CONTACT POINTS)
CANCER
> Signaling pathways
> Immune activation
> Tissue Structure
> Tumor penetration
DRUG SCREENING
REGENERATIVE MEDICINE
TOXICOLOGY
> Tox efficacy
Spheroids – Mesenchymal stem cells (MSCs) labeled with GFP after 72 hours.