Endocytosis assay
Improved observation of events taking place at the membrane
4DCELL DEVICE
READ-OUTS
Observation of non-adherent cells, detection of endocytosis events in adherent cells at the apical and basal membranes.
STANDARD CULTURE LIMITATION
Observing endocytosis events in non-adherent cells can be very challenging since cells cannot be easily observed in a common petri-dish.
Using TIRF microscopy on adherent cells, enables to study the mechanisms at the basal membrane however, the observation of apical membrane’s events is difficult.
ENDOCYTOSIS ASSAY
Using 4Dcell’s cell confinement, cells are confined between two surfaces enabling the study of non-adherent cells and an easier observation of the mechanisms at the membrane especially for clathrin-dependent endocytosis.
In addition, confining adherent cells enables the study of endocytosis mechanisms at the basal membrane, as usual, but also at the apical membrane (confinement membrane).
EXAMPLES
Non-adherent cells are forced to spread on the bottom of the well [1]
Cell confinement for TIRFM acquisition at basal and apical membranes
Formation of endocytic clathrin-coated structures on the plasma membrane of mammalian cells [2]
