> EXPLORE CELL MECHANICS
6-Wells static confiner – CSOW 610
The CSOW 610 – static confiner is a portable device that allows confining cells within two surfaces with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston.
The CSOW 610 is the device that controls the position of the pistons and it is compatible with several brands of 6-well plates. In the end of the confinement experiments, the cells can be retrieved enabling further biochemical studies. The user decides when and where to confine the cells as it is not necessary to plug it to any other device.
> User friendly and convenient
It is easy to assemble and was engineered to fit with several brands of 6-well plates
> Multiple confinement experiments simultaneously
Enables the study of different cells or to apply different confinement conditions at the same time
> Adaptable with your microscope
It was designed to fit in your microscope
> THE STATIC CELL CONFINER CSOW 610 IS COMPOSED BY:
1 x CSOW 610
12 x Confinement Piston in PDMS
12 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement
Available confinement heights – from 1, 2, 3, … up to 20 um (you can choose up to 3 heights to integrate your kit)
> IF YOU WANT TO MAKE YOUR SLIDES ADHESIVE TO YOUR CELLS OR NOT (OPTIONAL):
Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides
If this device does not fit with your constraints, get in touch with us! We can personalize it for you!
> SCHEME OF THE CSOW 610
Overall dimensions of the CSOW 610
The CSWO is compatible with different brands of 6-well plates (Cellvis, Falcon (glass and plastic bottom), Nunc, TPP)
> CANCER
Migration of metastatic cells
Cell contractility in mestastasis
DNA DSB repair (mechanically induced)
Genomic instability (cell division)
Separated co-culture
> IMMUNOLOGY
Migration of immune cells
Imaging of non-adhesive cells
> ORGAN PHYSIOLOGY
Migration of cancer cells
Cell differenciation with stiffness control
Wound healing
Separeted co-culture
Cell compression response
> RARE DISEASES
Cell nucleus integrity
> AGING
Cell nucleus integrity
Autophagy related diseases
> OBSERVATION OPTIMIZATION
Imaging of non-adhesive cells
Planar imaging of organelles
> FUNDAMENTAL RESEARCH
Cell volume (cell cycle)
Cell stretching response
Video of HeLa cells under confinement using a 4Dcell confiner, going from initial state to extremely confined.
Video of cells dividing under confinement using a 4Dcell confiner
Example of mammalian cells with before and after confinement images with fluorescent proteins
Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672
Actin flows induce a universal coupling between cell speed and cell persistence
P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386
Geometric friction directs cell migration
M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101
Mitotic rounding alters cell geometry to ensure efficient spindle assembly
O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283
Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414
Exploring the Function of Cell Shape and Size during Mitosis
C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169
Methods for Two-Dimensional Cell Confinement
M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29